Downstream scale-up purification of influenza virus using single-use bioprocessing equipment

This application note describes a case study where live influenza virus was purified using single-use bioprocessing technology. The GE Healthcare ReadyToProcess™ single-use platform is a flexible manufacturing platform which facilitates easy handling of virus solutions and helps improve productivity. As model system for a live, attenuated influenza virus vaccine, a A/H1N1/Solomon Island strain produced in Vero cells was used. Total process time for the downstream purification was 3 days and the overall scale-up process resulted in production of influenza virus with the same yield and purity as obtained in the laboratory scale.

Product Type: 
Single-use chromatography system and ReadyToProcess columns
Application Area: 
Cell culture scale-up / Expansion
Chromatography
Resource Type: 
Application notes
Sales Cycle: 
Exploration (Education)
Download 863.67 KB