Efficient, high-titer monoclonal antibody production in a fed-batch process using single-use stirred tank and rocking bioreactor systems

This work shows monoclonal antibody (MAb) production in parallel fed-batch processes using the single-use Xcellerex™ XDR-200 stirred-tank and ReadyToProcess WAVE™ 25 rocking bioreactor systems. Cell expansion was performed in a 15 L rocking bioreactor culture to seed a 200 L stirred- tank bioreactor culture. To compare the performance of ReadyToProcess WAVE 25 with that of XDR-200, 7 L of the 200 L culture was transferred to a parallel ReadyToProcess WAVE 25 system to a total culture volume of 10 L. This study showed that, although, having different vessel geometry, the ReadyToProcess WAVE 25 and the Xcellerex XDR-200 bioreactor were very comparable in performance and allowed for high performing fed-batch cultures.

Product Type: 
Bioreactors - Process development / seed train
Bioreactors - Production
Application Area: 
Bioreactor production
Resource Type: 
Sales Cycle: 
Evaluate (Persuasion)
Exploration (Education)
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