An intensified perfusion one-step seed process with high-density cell banks

This work describes how the use of perfusion in the seed culture expansion process, in combination with the use of high-density cell banks, drastically can reduce processing time, simplify operations, and maximize equipment utilization (Fig 1). A high-density cell bank was created using a method that does not require centrifugation. One vial from this cell bank was used to inoculate a bioreactor culture that, when operated in perfusion mode, enabled a one-step seed culture process that could be used to seed a 2000 L production bioreactor.

Product Type: 
Bioreactors - Process development / seed train
Application Area: 
Bioreactor production
Cell culture scale-up / Expansion
Resource Type: 
Sales Cycle: 
Evaluate (Persuasion)
Exploration (Education)
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